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HD-RNAS:Hierarchical Database of RNA Structures

A Classification Procedure for Functional RNA Crystal Structures

HD-RNAS is a classification of functional RNA structures available in PDB. All the RNA Crystallographic structures, NMR structures and Electron Microscopic structures having at least one RNA chain longer than 9 nucleotides are classified in 10 major functional classes. Each class is further classified according to the source organism from which the RNA molecules were isolated and crystallized.
Presently, HD-RNAS v2.0 contains 1060 crystal structures, containing 3066 RNA chains. The proper source organisms of RNA structure colected from sourse organism and other details field . The non-redundant set of structures consists of many PDB entries, indicating the best representative structures from each of the sub-classes based on their resolution and R-factor.

Structural/Functional Classification of 3066 RNA Chains

   mRNA

   Pseudoknot

   Ribonuclease

   Riboswitch

   Ribozyme

   rRNA

   snRNA

   tarRNA

   Tetraloop

   tRNA

   Unclassified


Search By Organism Name:

Enter Organism name: ([help]H. sapiens or Homo sapiens)
   

   


Search By Functional RNA Type:

(For class tRNA and rRNA, select the radiobutton first.) ([help]Only one option can be selected at a time. For class tRNA and rRNA, select the radiobutton first, then select the specisic type from the dropdown)

Transfer RNA (tRNA)
Ribosomal RNA (rRNA)
Messenger RNA (mRNA)
Ribonuclease
Riboswitch
Ribozyme
SRP RNA
Other-RNA



Search Sequence:

Paste your sequence here in FASTA format: ([help]GCGAUCGAUCGAUGCAAAAGCU
or >ACGACGACGAUUUUUAGCGA
)





Search By Author:

Enter Author Name: ([help]Enter name of the author like: Valle, M. )
   

   


Search By Resolution:

Enter Resolution in Angstrom: ([help]The value is in Angstrom. Minimum is 0.9. )

Resolution between
and
                              




Select or Remove search criteria from the dropdown below

([help]First choose the criteria from the drop down below, them to add as search criteria press the '+' button on the right. To remove a criteria select that criteria from dropdown and press the '-' button on the right. We recomend to use the 'refresh values' button below befor starting each of the search.)


Search Non-redundant database only

Search By Organism Name:

Enter Organism name: ([help]H. sapiens or Homo sapiens)
   


Search By Functional RNA Type:

(For class tRNA and rRNA, select the radiobutton first.) ([help]Only one option can be selected at a time. For class tRNA and rRNA, select the radiobutton first, then select the specisic type from the dropdown)

Transfer RNA (tRNA)
Ribosomal RNA (rRNA)
Messenger RNA (mRNA)
Ribonuclease
Riboswitch
Ribozyme
SRP RNA
Other-RNA



Search Sequence:

Paste your sequence here in FASTA format: ([help]GCGAUCGAUCGAUGCAAAAGCU
or >ACGACGACGAUUUUUAGCGA
)



Search By Author:

Enter Author Name: ([help]Enter name of the author like: Valle, M. )
   


Search By Resolution:

Enter Resolution in Angstrom: ([help]The value is in Angstrom. Minimum is 0.9. )

Resolution between and   Angstrom.



   


BPFIND


A Software for detection and analysis of Hydrogen Bonded Base Pairs in a nucleic acid structure. The program looks for at least two hydrogen bonds between two nucleotide bases. This can also detect possible protonated base pairs.


Citation : Das J., Mukherjee S., Mitra A., Bhattacharyya D. (2006). Non-canonical base pairs and higher order structures in nucleic acids crystal structure database analysis. J. Biomol. Struct. Dynam. 24, 149–161 [PubMed]

NUPARM

A Software for analysis of nucleic acid structure mainly through different base pair parameters.


Citation : Mukherjee S., Bansal M., Bhattacharyya D. (2006). Conformational specificity of non-canonical base pairs and higher order structures in nucleic acids: crystal structure database analysis. J. Comput.Aided Mol.Des. 20 (10-11), 629-645 [PubMed]

Frequently Asked Questions

Q1. I am using PDB ids like "1GTR.pdb, 1Y77.pdb " in ID Search but why it is not working?

Ans. The ID search accepts pdb ids not pdb files. One should use ids like "1GTR, 1Y77". Do not use the ".pdb" part.
Q2. How to download in batch file?

Ans. Press the Batch Download button first. Browse for file containing PDB id and Molecule id information in proper format. Click download button. The format should be like:
1GTR, 1, 2
3PIO, 1, 2, 3, 4, 5, 6

Tutorial

Search Database


One can search the database with respect to various parameters. Search related specific help are given in particular search types for more conveniance.





  • PDB/RCSB/NDB/BMRB ids can be used for search criteria. Multiple searches are catered while ids are seperated by comma (,). Use button after entering the id . Wrong typping can be cleared using the button
    Search Database > PDB/RCSB/NDB ids
  • Search By PDB-ID / NDB-ID / RCSB-ID:

    Enter ID: ([help]PDB or RCSB or NDB or BMRB ids can be used as search criteria. More than one id can be used seperating by ",”
    Single : GTR
    Multiple : 1GTR, 1JJ2
    )
       

       


  • Organism Search process is very similar to that of ID Search. In organism search one can simply query with Genus species or G. species type of query. Only one organism at a time can be search here.
  • Some well known functional RNA types are pre determined for Functional RNA Search catagory. One has to select the radio button on left of a particular catagory to search that. In case of rRNA/tRNA first select the radio button on left, then select specific type of rRNA/tRNA from dropdown on the right.
    Search Database > Functional RNA Types
  • RNA Sequences Search process tries to search full or part of RNA sequences within this database. No '>' notation or header regions are accepted, only sequences are to be given for search.
  • Crystal structures can be searched using time period criteria in Year Search. Molecules that are being deposited within this time period are shown in this process. Time period can be selected from pop-up calender or can be given in format of MM/DD/YYYY



  • Advanced Search


    Database can be searched using various combination of search in Advance Search.



  • search criteria can be selected using dropdown and buttons with adds new criteria where removes the criteria
  • Non-redundant dataset can be searched using same criterias. One have to check the box on left of Search Non-redundant database only

  • Download

    ([help]Add PDB id and molecule id separated by comma (,); For Multiple Entry seperate PDB ids by comma(,) and respective molecule ids by semicolon (;))
    PDB id         

    Molecule id

    Fasta sequences

    File

    Authors: Debasish Mukherjee, Dhananjay Bhattacharyya
    Last modified on: 15-Jul-2013 | For details of classification procedure click here | Please send your bug reports to: dhananjay.bhattacharyya[AT]saha.ac.in