tparam : santa Na : 0.165 K : 0.00 NH4 : =0.00 Mg : 0.01 Mono : 0.165 OUTPUT

Results

The input sequence has passed the validity test


Your sequence tag : gi|151936653|gb|EF687843.1|EF687843 PLN Arabidopsis thaliana ecotype C24 BABY BOOM (BBM) mRNA, complete cds.
Length of your input sequence : 1755
Your chosen Temperature : 37 deg C
Your chosen database of base pair step parameter : Santalucia, PNAS, 1998
Your chosen Monovalent Counterion Concentration : 0.165 (M)
Your chosen Divalent Counterion Concentration : 0.01 (M)
Window-size : 10 bp & Overlap : 0 bp

[Press Ctrl and select any column using mouse]

wseq_num delta H0 (Kcal/mol) delta S0 (cal/mol/K) delta G0(T) (Kcal/mol) delta G0(37) (Kcal/mol) Tm (stacking, [Mon+] corrected, santalucia '98) (degree C) Tm (stacking, [Mon+] & [Mg+2] corrected, owczarzy '08) (degree C) Tm (%GC-based) (degree C)
wseq:1
-75.000
-209.794
-9.935
-9.861
84.344
84.580
28.737
wseq:2
-71.600
-204.394
-8.210
-8.121
77.154
79.139
24.637
wseq:3
-73.000
-203.894
-9.765
-9.701
84.880
85.307
32.837
wseq:4
-72.200
-202.294
-9.461
-9.361
83.757
84.508
32.837
wseq:5
-72.300
-203.794
-9.096
-9.031
81.620
82.734
28.737
wseq:6
-72.000
-204.694
-8.517
-8.441
78.595
80.274
24.637
wseq:7
-77.100
-212.994
-11.043
-11.011
88.832
87.958
36.937
wseq:8
-73.000
-203.094
-10.013
-9.931
86.290
86.451
32.837
wseq:9
-74.300
-203.194
-11.282
-11.231
92.511
91.275
36.937
wseq:10
-78.800
-212.994
-12.743
-12.741
96.814
94.096
41.037
wseq:11
-76.900
-209.094
-12.052
-12.051
94.628
92.600
36.937
wseq:12
-72.500
-200.294
-10.382
-10.331
88.818
88.562
32.837
wseq:13
-72.200
-202.794
-9.306
-9.241
82.877
83.776
28.737
wseq:14
-76.400
-204.994
-12.824
-12.831
99.544
96.568
45.137
wseq:15
-73.800
-207.794
-9.355
-9.301
82.010
82.841
28.737
wseq:16
-77.400
-211.494
-11.808
-11.761
92.818
91.101
36.937
wseq:17
-71.700
-204.794
-8.186
-8.091
76.958
78.949
20.537
wseq:18
-71.800
-201.194
-9.402
-9.321
83.720
84.522
28.737
wseq:19
-76.700
-211.194
-11.201
-11.131
90.024
88.948
32.837
wseq:20
-76.300
-207.294
-12.011
-11.991
94.927
92.934
41.037
wseq:21
-73.400
-204.794
-9.886
-9.801
85.259
85.543
28.737
wseq:22
-69.700
-202.394
-6.930
-6.851
71.228
74.451
20.537
wseq:23
-76.100
-207.194
-11.842
-11.801
94.139
92.338
41.037
wseq:24
-72.200
-200.194
-10.113
-10.071
87.501
87.545
32.837
wseq:25
-72.400
-202.894
-9.475
-9.431
83.687
84.408
28.737
wseq:26
-77.100
-211.894
-11.384
-11.341
90.712
89.446
32.837
wseq:27
-70.600
-204.394
-7.210
-7.111
72.262
75.187
20.537
wseq:28
-73.700
-209.794
-8.635
-8.551
78.147
79.665
24.637
wseq:29
-72.000
-201.394
-9.540
-9.491
84.359
85.013
28.737
wseq:30
-73.600
-206.194
-9.652
-9.571
83.796
84.326
28.737
wseq:31
-73.700
-206.794
-9.566
-9.511
83.244
83.862
28.737
wseq:32
-80.000
-213.294
-13.850
-13.851
101.920
97.949
45.137
wseq:33
-74.300
-210.294
-9.080
-9.001
80.165
81.260
28.737
wseq:34
-74.100
-207.994
-9.593
-9.541
83.111
83.698
28.737
wseq:35
-76.200
-209.694
-11.166
-11.101
90.237
89.185
32.837
wseq:36
-70.900
-200.494
-8.720
-8.671
80.477
81.986
24.637
wseq:37
-77.100
-211.394
-11.539
-11.511
91.572
90.149
36.937
wseq:38
-72.200
-198.894
-10.516
-10.461
89.858
89.458
36.937
wseq:39
-76.800
-206.794
-12.666
-12.651
98.235
95.470
41.037
wseq:40
-79.100
-211.394
-13.539
-13.541
101.033
97.374
45.137
wseq:41
-72.600
-201.394
-10.140
-10.101
87.338
87.355
32.837
wseq:42
-70.200
-203.094
-7.213
-7.131
72.503
75.449
20.537
wseq:43
-74.900
-205.694
-11.107
-11.071
90.984
89.957
32.837
wseq:44
-70.700
-203.294
-7.651
-7.571
74.623
77.163
24.637
wseq:45
-72.700
-203.794
-9.496
-9.451
83.583
84.280
28.737
wseq:46
-72.000
-204.094
-8.703
-8.651
79.629
81.140
28.737
wseq:47
-72.400
-202.994
-9.444
-9.381
83.511
84.264
28.737
wseq:48
-77.300
-212.494
-11.398
-11.351
90.625
89.351
32.837
wseq:49
-77.000
-210.294
-11.780
-11.741
93.005
91.301
36.937
wseq:50
-70.200
-196.194
-9.353
-9.321
84.660
85.543
32.837
wseq:51
-71.100
-202.794
-8.206
-8.131
77.453
79.459
24.637
wseq:52
-70.700
-201.994
-8.054
-7.971
76.861
79.028
24.637
wseq:53
-74.400
-208.594
-9.707
-9.621
83.524
83.994
28.737
wseq:54
-73.200
-207.194
-8.942
-8.851
80.143
81.379
24.637
wseq:55
-75.000
-209.694
-9.966
-9.911
84.514
84.733
32.837
wseq:56
-72.500
-202.894
-9.575
-9.491
84.180
84.795
28.737
wseq:57
-71.500
-204.094
-8.203
-8.111
77.179
79.174
24.637
wseq:58
-74.500
-209.094
-9.652
-9.581
83.150
83.690
32.837
wseq:59
-72.500
-203.794
-9.296
-9.211
82.602
83.508
28.737
wseq:60
-72.000
-204.894
-8.455
-8.341
78.252
79.990
24.637
wseq:61
-71.900
-203.594
-8.758
-8.671
80.004
81.450
24.637
wseq:62
-73.100
-204.794
-9.586
-9.501
83.795
84.395
28.737
wseq:63
-73.200
-207.694
-8.786
-8.711
79.292
80.693
28.737
wseq:64
-79.500
-214.694
-12.915
-12.911
97.145
94.268
41.037
wseq:65
-74.000
-206.094
-10.083
-10.021
85.910
85.986
28.737
wseq:66
-76.800
-208.894
-12.014
-11.971
94.501
92.513
36.937
wseq:67
-73.000
-205.394
-9.300
-9.251
82.265
83.176
32.837
wseq:68
-74.000
-205.194
-10.362
-10.321
87.485
87.258
28.737
wseq:69
-71.900
-200.094
-9.844
-9.821
86.182
86.507
28.737
wseq:70
-74.600
-209.294
-9.690
-9.601
83.287
83.759
24.637
wseq:71
-74.200
-202.794
-11.306
-11.271
92.739
91.471
36.937
wseq:72
-77.300
-213.794
-10.995
-10.921
88.413
87.580
32.837
wseq:73
-72.700
-203.094
-9.713
-9.631
84.813
85.296
32.837
wseq:74
-70.700
-199.094
-8.954
-8.911
81.959
83.247
28.737
wseq:75
-71.600
-201.794
-9.016
-8.951
81.668
82.874
28.737
wseq:76
-71.500
-204.694
-8.017
-7.901
76.152
78.309
20.537
wseq:77
-74.400
-206.294
-10.421
-10.371
87.501
87.232
32.837
wseq:78
-75.100
-208.294
-10.500
-10.471
87.399
87.053
32.837
wseq:79
-78.000
-212.794
-12.005
-11.981
93.402
91.487
36.937
wseq:80
-70.000
-202.994
-7.044
-6.951
71.688
74.794
20.537
wseq:81
-73.400
-198.794
-11.747
-11.731
96.077
94.248
41.037
wseq:82
-70.200
-201.394
-7.740
-7.641
75.421
77.904
24.637
wseq:83
-72.000
-197.994
-10.595
-10.561
90.498
89.984
32.837
wseq:84
-70.600
-202.094
-7.923
-7.851
76.193
78.487
24.637
wseq:85
-71.800
-201.194
-9.402
-9.321
83.720
84.537
32.837
wseq:86
-70.300
-200.794
-8.027
-7.941
76.961
79.170
24.637
wseq:87
-74.800
-207.994
-10.293
-10.231
86.476
86.350
32.837
wseq:88
-77.000
-210.294
-11.780
-11.741
93.005
91.301
36.937
wseq:89
-73.700
-206.794
-9.566
-9.501
83.244
83.862
28.737
wseq:90
-78.500
-213.394
-12.319
-12.281
94.715
92.480
41.037
wseq:91
-71.100
-201.694
-8.547
-8.461
79.365
81.040
24.637
wseq:92
-73.300
-204.494
-9.879
-9.791
85.296
85.603
32.837
wseq:93
-78.900
-213.894
-12.564
-12.521
95.725
93.208
36.937
wseq:94
-72.600
-207.594
-8.217
-8.151
76.572
78.513
24.637
wseq:95
-71.900
-201.894
-9.285
-9.201
82.978
83.917
32.837
wseq:96
-74.300
-205.194
-10.662
-10.621
88.947
88.409
32.837
wseq:97
-73.100
-204.694
-9.617
-9.551
83.969
84.537
28.737
wseq:98
-76.700
-206.894
-12.535
-12.521
97.572
94.964
41.037
wseq:99
-73.400
-203.594
-10.258
-10.231
87.372
87.268
32.837
wseq:100
-79.400
-214.794
-12.784
-12.761
96.507
93.779
41.037
wseq:101
-72.200
-205.894
-8.345
-8.231
77.516
79.353
24.637
wseq:102
-71.600
-200.994
-9.264
-9.191
83.080
84.044
32.837
wseq:103
-74.900
-208.794
-10.145
-10.071
85.577
85.609
32.837
wseq:104
-76.100
-207.694
-11.686
-11.631
93.255
91.619
36.937
wseq:105
-75.500
-204.894
-11.955
-11.931
95.334
93.346
36.937
wseq:106
-72.400
-202.394
-9.630
-9.591
84.569
85.140
32.837
wseq:107
-76.400
-210.194
-11.211
-11.171
90.324
89.228
32.837
wseq:108
-69.900
-203.094
-6.913
-6.831
71.026
74.250
20.537
wseq:109
-70.200
-199.694
-8.268
-8.201
78.388
80.368
24.637
wseq:110
-80.000
-216.694
-12.795
-12.751
96.035
93.334
41.037
wseq:111
-74.600
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-9.349
-9.251
81.423
82.251
28.737
wseq:112
-71.900
-203.494
-8.789
-8.721
80.178
81.593
24.637
wseq:113
-71.900
-205.794
-8.076
-7.981
76.229
78.315
20.537
wseq:114
-73.400
-203.294
-10.351
-10.311
87.904
87.697
32.837
wseq:115
-76.600
-207.294
-12.311
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96.374
94.036
41.037
wseq:116
-70.200
-199.194
-8.423
-8.361
79.271
81.111
28.737
wseq:117
-72.200
-202.394
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83.580
84.350
28.737
wseq:118
-80.000
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-11.865
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90.993
89.325
36.937
wseq:119
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89.629
88.725
32.837
wseq:120
-71.400
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76.176
78.355
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wseq:121
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86.939
86.890
32.837
wseq:122
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76.169
78.453
24.637
wseq:123
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74.874
77.074
20.537
wseq:124
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-9.917
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85.435
85.701
32.837
wseq:125
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-7.421
73.769
76.437
20.537
wseq:126
-76.200
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92.680
91.149
36.937
wseq:127
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-9.355
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82.010
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28.737
wseq:128
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88.970
87.988
32.837
wseq:129
-72.600
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88.956
88.659
32.837
wseq:130
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-8.841
80.966
82.284
24.637
wseq:131
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84.483
84.747
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wseq:132
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90.611
36.937
wseq:133
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87.422
87.156
36.937
wseq:134
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74.558
77.171
20.537
wseq:135
-72.300
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-10.120
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87.460
87.497
32.837
wseq:136
-72.100
-204.694
-8.617
-8.511
79.084
80.663
24.637
wseq:137
-74.600
-206.894
-10.435
-10.371
87.422
87.156
36.937
wseq:138
-72.100
-201.294
-9.671
-9.611
85.033
85.546
28.737
wseq:139
-73.000
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-8.773
-8.661
79.347
80.752
24.637
wseq:140
-72.800
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-10.527
-10.471
89.411
88.995
32.837
wseq:141
-74.000
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-9.649
-9.541
83.487
84.019
28.737
wseq:142
-72.600
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-8.791
80.317
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24.637
wseq:143
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wseq:144
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wseq:145
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wseq:146
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wseq:147
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wseq:148
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wseq:149
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wseq:150
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wseq:151
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wseq:152
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wseq:153
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wseq:154
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wseq:155
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wseq:156
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wseq:157
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wseq:158
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wseq:159
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88.970
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32.837
wseq:160
-69.200
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70.626
74.007
16.437
wseq:161
-73.400
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84.212
84.693
28.737
wseq:162
-76.200
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89.718
88.769
32.837
wseq:163
-75.400
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-10.707
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88.318
87.753
32.837
wseq:164
-75.100
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-10.562
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87.745
87.333
32.837
wseq:165
-76.600
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-13.365
-13.391
102.536
98.868
45.137
wseq:166
-73.900
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-10.107
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86.122
86.187
32.837
wseq:167
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wseq:168
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wseq:169
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84.039
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wseq:170
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-8.790
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79.574
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24.637
wseq:171
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97.837
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wseq:172
-72.800
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89.411
89.012
36.937
wseq:173
-74.600
-201.894
-11.985
-11.971
96.351
94.292
41.037
wseq:174
-72.900
-206.794
-8.766
-8.651
79.375
80.789
24.637
wseq:175
-76.700
-211.194
-11.201
-11.131
90.024
88.948
32.837
wseq:176
-30.700
-92.794
-1.923
-1.881
57.691
77.069
-31.263

Tm (stacking-based) (deg C)


84.580
79.139
85.307
84.508
82.734
80.274
87.958
86.451
91.275
94.096
92.600
88.562
83.776
96.568
82.841
91.101
78.949
84.522
88.948
92.934
85.543
74.451
92.338
87.545
84.408
89.446
75.187
79.665
85.013
84.326
83.862
97.949
81.260
83.698
89.185
81.986
90.149
89.458
95.470
97.374
87.355
75.449
89.957
77.163
84.280
81.140
84.264
89.351
91.301
85.543
79.459
79.028
83.994
81.379
84.733
84.795
79.174
83.690
83.508
79.990
81.450
84.395
80.693
94.268
85.986
92.513
83.176
87.258
86.507
83.759
91.471
87.580
85.296
83.247
82.874
78.309
87.232
87.053
91.487
74.794
94.248
77.904
89.984
78.487
84.537
79.170
86.350
91.301
83.862
92.480
81.040
85.603
93.208
78.513
83.917
88.409
84.537
94.964
87.268
93.779
79.353
84.044
85.609
91.619
93.346
85.140
89.228
74.250
80.368
93.334
82.251
81.593
78.315
87.697
94.036
81.111
84.350
89.325
88.725
78.355
86.890
78.453
77.074
85.701
76.437
91.149
82.841
87.988
88.659
82.284
84.747
90.611
87.156
77.171
87.497
80.663
87.156
85.546
80.752
88.995
84.019
81.607
93.953
78.957
81.453
85.896
82.442
86.262
83.066
81.454
85.691
81.986
91.951
84.580
79.036
87.509
91.906
81.439
87.988
74.007
84.693
88.769
87.753
87.333
98.868
86.187
88.170
79.676
84.551
80.967
95.419
89.012
94.292
80.789
88.948
77.069

delG0(37) (Kcal/mol)


-9.861
-8.121
-9.701
-9.361
-9.031
-8.441
-11.011
-9.931
-11.231
-12.741
-12.051
-10.331
-9.241
-12.831
-9.301
-11.761
-8.091
-9.321
-11.131
-11.991
-9.801
-6.851
-11.801
-10.071
-9.431
-11.341
-7.111
-8.551
-9.491
-9.571
-9.511
-13.851
-9.001
-9.541
-11.101
-8.671
-11.511
-10.461
-12.651
-13.541
-10.101
-7.131
-11.071
-7.571
-9.451
-8.651
-9.381
-11.351
-11.741
-9.321
-8.131
-7.971
-9.621
-8.851
-9.911
-9.491
-8.111
-9.581
-9.211
-8.341
-8.671
-9.501
-8.711
-12.911
-10.021
-11.971
-9.251
-10.321
-9.821
-9.601
-11.271
-10.921
-9.631
-8.911
-8.951
-7.901
-10.371
-10.471
-11.981
-6.951
-11.731
-7.641
-10.561
-7.851
-9.321
-7.941
-10.231
-11.741
-9.501
-12.281
-8.461
-9.791
-12.521
-8.151
-9.201
-10.621
-9.551
-12.521
-10.231
-12.761
-8.231
-9.191
-10.071
-11.631
-11.931
-9.591
-11.171
-6.831
-8.201
-12.751
-9.251
-8.721
-7.981
-10.311
-12.291
-8.361
-9.351
-11.801
-10.961
-7.971
-10.151
-7.861
-7.831
-9.821
-7.421
-11.581
-9.251
-11.071
-10.381
-8.841
-9.861
-11.571
-10.371
-7.511
-10.081
-8.511
-10.371
-9.611
-8.661
-10.471
-9.541
-8.791
-12.931
-8.001
-8.741
-10.211
-8.861
-10.241
-9.221
-8.821
-9.661
-8.671
-11.741
-9.541
-8.231
-10.351
-11.851
-8.751
-11.071
-6.711
-9.641
-11.031
-10.631
-10.481
-13.391
-10.071
-10.921
-8.111
-9.591
-8.681
-12.291
-10.471
-11.971
-8.651
-11.131
-1.881

Window-Sequences


wseq sense strand sequence (5`->3`) length (bp)
wseq:1
ATGAACTCGA
10
wseq:2
TGAATAACTG
10
wseq:3
GTTAGGCTTC
10
wseq:4
TCTCTCTCTC
10
wseq:5
CTCATGATCA
10
wseq:6
AAATCATCAC
10
wseq:7
CGTACGGATG
10
wseq:8
TTGACTCCTC
10
wseq:9
CACCACCAGA
10
wseq:10
ACCGCCGTAG
10
wseq:11
ATGTTGCCGG
10
wseq:12
AGGGTACTGT
10
wseq:13
TTTGATCTGG
10
wseq:14
CCGCTCCCTC
10
wseq:15
CGATGAATCT
10
wseq:16
TCTGCCGTTC
10
wseq:17
AAACATCTTT
10
wseq:18
TCTTTCTCCT
10
wseq:19
TTCGGTGTCA
10
wseq:20
CCCTCGAAGC
10
wseq:21
TTTCACCAGA
10
wseq:22
GACAATAATA
10
wseq:23
GTCACTCCCG
10
wseq:24
AGATTGGGAC
10
wseq:25
ATCAATGGTG
10
wseq:26
GTGCATGCAA
10
wseq:27
TAACATTAAC
10
wseq:28
AATAACGAAC
10
wseq:29
AAAATGGACC
10
wseq:30
AAAGCTTGAG
10
wseq:31
AATTTCCTCG
10
wseq:32
GCCGCACCAC
10
wseq:33
CACGATTTAC
10
wseq:34
AATACCAACG
10
wseq:35
AGACCGTTGT
10
wseq:36
AGATGGAAAT
10
wseq:37
GGCGATTGTG
10
wseq:38
GAGGAGGAGA
10
wseq:39
CGGTGGTGGT
10
wseq:40
GGCGGCTCAC
10
wseq:41
TAGGCCTTTC
10
wseq:42
GATGATAAAA
10
wseq:43
ACATGGCTGA
10
wseq:44
GTAATCATTC
10
wseq:45
GGTTGCTAAT
10
wseq:46
GCTAATCATC
10
wseq:47
AAGACAATGG
10
wseq:48
TAACGGTGCA
10
wseq:49
CGAGGCTTGT
10
wseq:50
CCCTCTCTAT
10
wseq:51
GAATTCATCT
10
wseq:52
ACTAGTGATA
10
wseq:53
GCAACAACTA
10
wseq:54
CAACAACAAT
10
wseq:55
GATGATGTCG
10
wseq:56
TCCAAGAGAA
10
wseq:57
GACTATTGTT
10
wseq:58
GATGTCGTAG
10
wseq:59
AAACTACACC
10
wseq:60
GAAGAAAACT
10
wseq:61
ATTGAGAGTT
10
wseq:62
TTGGACAAAG
10
wseq:63
GACGTCTATA
10
wseq:64
TACCGCGGTG
10
wseq:65
TTACAAGGCA
10
wseq:66
TCGGTGGACA
10
wseq:67
GGTAGATACG
10
wseq:68
AGGCACATTT
10
wseq:69
ATGGGACAAT
10
wseq:70
AGTTGCAAAA
10
wseq:71
GAGAAGGCCA
10
wseq:72
GACTCGCAAA
10
wseq:73
GGAAGACAAG
10
wseq:74
TTTATCTGGG
10
wseq:75
AGGTTATGAC
10
wseq:76
AAAGAAGAAA
10
wseq:77
AAGCAGCTAG
10
wseq:78
GGCTTACGAT
10
wseq:79
TTAGCCGCAC
10
wseq:80
TAAAGTATTG
10
wseq:81
GGGAACCACC
10
wseq:82
ACTACTACTA
10
wseq:83
ACTTCCCCTT
10
wseq:84
GAGTGAATAT
10
wseq:85
GAGAAAGAGG
10
wseq:86
TAGAAGAGAT
10
wseq:87
GAAGCACATG
10
wseq:88
ACGAGGCAAG
10
wseq:89
AGTATGTTGC
10
wseq:90
CTCTCTGCGC
10
wseq:91
AGGAAAAGTA
10
wseq:92
GTGGTTTCTC
10
wseq:93
TCGTGGTGCA
10
wseq:94
TCGATTTATC
10
wseq:95
GAGGAGTAAC
10
wseq:96
AAGGCATCAC
10
wseq:97
CAACATGGAA
10
wseq:98
GGTGGCAAGC
10
wseq:99
TAGGATCGGA
10
wseq:100
AGAGTCGCCG
10
wseq:101
GTAACAAAGA
10
wseq:102
CCTCTACTTG
10
wseq:103
GGAACTTTCG
10
wseq:104
GCACACAGGA
10
wseq:105
AGAGGCTGCT
10
wseq:106
GAGGCTTATG
10
wseq:107
ACATTGCAGC
10
wseq:108
CATTAAATTC
10
wseq:109
AGAGGATTAA
10
wseq:110
GCGCAGTGAC
10
wseq:111
TAACTTCGAC
10
wseq:112
ATGAACAGAT
10
wseq:113
ACAATGTTAA
10
wseq:114
AGCAATCCTC
10
wseq:115
GAGAGCCCGA
10
wseq:116
GTCTACCTAT
10
wseq:117
TGGTAGTTCT
10
wseq:118
GCGAAACGTC
10
wseq:119
TCAAGGACGT
10
wseq:120
TAATAATCCG
10
wseq:121
GTTCCAGCTA
10
wseq:122
TGATGATTAG
10
wseq:123
TAATAACGTT
10
wseq:124
TCAGAGAGTG
10
wseq:125
CAAATAATGT
10
wseq:126
TAGCGGTTGG
10
wseq:127
CAAAACACTG
10
wseq:128
CGTTTCAGCA
10
wseq:129
TCATCAGGGA
10
wseq:130
ATGGATTTGA
10
wseq:131
GCTTATTGCA
10
wseq:132
GCAACAGCAG
10
wseq:133
GAGAGGTACG
10
wseq:134
TTGGTTATTA
10
wseq:135
CAATGGAGGA
10
wseq:136
AACTTGTCTA
10
wseq:137
CCGAGAGTAC
10
wseq:138
TAGGGTTTGT
10
wseq:139
TTCAAACAAG
10
wseq:140
AGGAGGAACA
10
wseq:141
ACAACACTTC
10
wseq:142
TTGAGAAACT
10
wseq:143
CGCCGAGTCA
10
wseq:144
CATGACTAAT
10
wseq:145
GTTGATCATC
10
wseq:146
ATAGCTCGAC
10
wseq:147
CTCTGATGAT
10
wseq:148
TCTGTTACCG
10
wseq:149
TTTGTGGAAA
10
wseq:150
TGTTGTTAGT
10
wseq:151
TATGGTGGTT
10
wseq:152
ATCAAGGATT
10
wseq:153
CGCAATCCCT
10
wseq:154
GTTGGAACAT
10
wseq:155
CGGTTAATTA
10
wseq:156
CGATCCCTTT
10
wseq:157
ACTGCTGCTG
10
wseq:158
AGATTGCTTA
10
wseq:159
CAACGCAAGA
10
wseq:160
AATCATTATT
10
wseq:161
ACTATGCTCA
10
wseq:162
GCATCAGCAA
10
wseq:163
CAACAGCAGA
10
wseq:164
TTCAGCAGTC
10
wseq:165
GCCGGGAGGA
10
wseq:166
GATTTTCCGG
10
wseq:167
TGGCGATTTC
10
wseq:168
GAATAACCAT
10
wseq:169
AGCTCTAACA
10
wseq:170
TGTACTTTCA
10
wseq:171
CGGGGAAGGT
10
wseq:172
GGTGGAGAAG
10
wseq:173
GGGCTCCAAC
10
wseq:174
GTTTTCAGTT
10
wseq:175
TGGAACGACA
10
wseq:176
CTTAG
5

Global Melting Origin


Global Melting origin identified at wseq:160 (Tm = 74.007 deg C; delG0(37) = -6.711 Kcal/mol)
3`-most wseq was not considered since it was shorter (5 bp) than the window-size specified by the user (10 bp)

Local minima:


wseq_num
Tm(stacking) (deg C)
delG0(37) (Kcal/mol)
wseq:2
79.139
-8.121
wseq:6
80.274
-8.441
wseq:8
86.451
-9.931
wseq:13
83.776
-9.241
wseq:15
82.841
-9.301
wseq:17
78.949
-8.091
wseq:22
74.451
-6.851
wseq:25
84.408
-9.431
wseq:27
75.187
-7.111
wseq:31
83.862
-9.511
wseq:33
81.260
-9.001
wseq:36
81.986
-8.671
wseq:38
89.458
-10.461
wseq:42
75.449
-7.131
wseq:44
77.163
-7.571
wseq:46
81.140
-8.651
wseq:52
79.028
-7.971
wseq:54
81.379
-8.851
wseq:57
79.174
-8.111
wseq:60
79.990
-8.341
wseq:63
80.693
-8.711
wseq:65
85.986
-10.021
wseq:67
83.176
-9.251
wseq:70
83.759
-9.601
wseq:76
78.309
-7.901
wseq:78
87.053
-10.471
wseq:80
74.794
-6.951
wseq:82
77.904
-7.641
wseq:84
78.487
-7.851
wseq:86
79.170
-7.941
wseq:89
83.862
-9.501
wseq:91
81.040
-8.461
wseq:94
78.513
-8.151
wseq:97
84.537
-9.551
wseq:99
87.268
-10.231
wseq:101
79.353
-8.231
wseq:106
85.140
-9.591
wseq:108
74.250
-6.831
wseq:113
78.315
-7.981
wseq:116
81.111
-8.361
wseq:120
78.355
-7.971
wseq:123
77.074
-7.831
wseq:125
76.437
-7.421
wseq:127
82.841
-9.251
wseq:130
82.284
-8.841
wseq:134
77.171
-7.511
wseq:136
80.663
-8.511
wseq:139
80.752
-8.661
wseq:142
81.607
-8.791
wseq:144
78.957
-8.001
wseq:147
82.442
-8.861
wseq:150
81.454
-8.821
wseq:152
81.986
-8.671
wseq:155
79.036
-8.231
wseq:158
81.439
-8.751
wseq:164
87.333
-10.481
wseq:166
86.187
-10.071
wseq:168
79.676
-8.111
wseq:170
80.967
-8.681
wseq:172
89.012
-10.471
wseq:174
80.789
-8.651
wseq:176
77.069
-1.881